Scheie P
Biochim Biophys Acta. 1982 Jun 16;716(3):420-3. doi: 10.1016/0304-4165(82)90035-6.
Lysozyme attacked Escherichia coli B/r in the absence of EDTA or imposed osmotic shocks when the cells were rapidly cooled below specific temperatures. Cells subjected to lysozyme while being cooled to below 20 degrees C began to lose ability to subsequently form colonies. This sensitivity increased with decreasing temperatures and almost all cells cooled to 0 degrees C were affected. Slightly hypertonic solutions did not improve survival. Cells cooled first to as low as 5 degrees C and then subjected to lysozyme while cool did not lose their ability to form colonies subsequent to rewarming. However, 70% of the cells cooled first to 0 degrees C and subjected to lysozyme lost their colony-forming ability. Cell lysis also began when treated near 5 degrees C, but even when treated at 0 degrees C about 50% of the cells maintained their rod shape in the presence of lysozyme. These results are discussed in terms of a possible phase transition in a portion of the cell envelope and/or a transient osmotic swelling as a results of metabolic pumps failing at the low temperatures.
在没有乙二胺四乙酸（EDTA）的情况下，溶菌酶可攻击大肠杆菌B/r，或者当细胞迅速冷却至特定温度以下时施加渗透压冲击。在冷却至20摄氏度以下时受到溶菌酶作用的细胞开始丧失随后形成菌落的能力。这种敏感性随着温度降低而增加，几乎所有冷却至0摄氏度的细胞都受到影响。略高渗的溶液并不能提高存活率。先冷却至低至5摄氏度然后在冷却时受到溶菌酶作用的细胞在复温后不会丧失形成菌落的能力。然而，先冷却至0摄氏度然后受到溶菌酶作用的细胞中有70%丧失了菌落形成能力。在接近5摄氏度处理时细胞裂解也会开始，但即使在0摄氏度处理，在溶菌酶存在的情况下约50%的细胞仍保持杆状形态。这些结果从细胞包膜的一部分可能发生相变和/或由于低温下代谢泵失效导致的短暂渗透性肿胀方面进行了讨论。
