Magdalou J, Antoine B, Ratanasavanh D, Siest G
Enzyme. 1982;28(1):41-7. doi: 10.1159/000459083.
The presence of cytochrome P-450 and UDP-glucuronosyltransferase was confirmed in rabbit liver plasma membranes. Their levels, expressed as amount or activity/mg protein, were 25 and 40% respectively of those found in hepatic microsomes. Both enzymes were induced by phenobarbital treatment of the animals: administration of the barbiturate for 4 days, produced a 150% increase in the cytochrome P-450 content and in the specific activity of UDP-glucuronosyltransferase. As compared with the microsomal enzymes, the plasma membrane enzymes were localized in a lipid environment characterized by high levels of cholesterol and sphingomyelin and a low level of phosphatidylcholine. Treatment with phenobarbital for 4 days also caused a significant increase of phosphatidylcholine and phosphatidylethanolamine content in plasma membranes.
在兔肝细胞膜中证实存在细胞色素P - 450和UDP - 葡萄糖醛酸基转移酶。以每毫克蛋白质的含量或活性表示，它们的水平分别为肝微粒体中相应水平的25%和40%。用苯巴比妥处理动物可诱导这两种酶：给予巴比妥酸盐4天，细胞色素P - 450含量和UDP - 葡萄糖醛酸基转移酶的比活性增加了150%。与微粒体酶相比，细胞膜酶定位于以高水平胆固醇和鞘磷脂以及低水平磷脂酰胆碱为特征的脂质环境中。用苯巴比妥处理4天也导致细胞膜中磷脂酰胆碱和磷脂酰乙醇胺含量显著增加。
