对4'-[(9-吖啶基)-氨基]甲磺酰基间茴香胺作用机制的重新评估。

A reassessment of the mechanism of action of 4'-[(9-acridinyl)-amino]methanesulphon-m-anisidide.

作者信息

Marshall B, Ralph R K

出版信息

Eur J Cancer Clin Oncol. 1982 Jun;18(6):553-7. doi: 10.1016/0277-5379(82)90224-3.

Abstract

The conclusion that treating cells with 4'-[(9-acridinyl)- amino]methanesulphon-m-anisidide (mAMSA) causes an alkali-dependent decrease in the size of their DNA was reinvestigated. Evidence is presented that alkali is not needed to detect an effect of mAMSA on the sedimentation rate of DNA released from mAMSA-treated PY815 cells and that mAMSA causes double-strand breaks in cellular DNA.

摘要

关于用4'-[(9-吖啶基)-氨基]甲磺基间茴香胺（mAMSA）处理细胞会导致其DNA大小呈碱依赖性减小这一结论，我们进行了重新研究。有证据表明，检测mAMSA对从经mAMSA处理的PY815细胞中释放的DNA沉降速率的影响并不需要碱，而且mAMSA会导致细胞DNA双链断裂。