White J L, Dawson W O
Intervirology. 1978;10(4):221-7. doi: 10.1159/000148985.
An examination of the effect of supraoptimal temperatures upon tobacco mosaic virus (TMV) RNA replicase demonstrated that the enzyme activity rapidly decreased at 40 degrees whether incubated in vitro after extraction or in leaves at that temperature prior to extraction and assay. The loss of replicase activity paralled the decline in capacity for in vivo incorporation of 32P into TMV RNA at 25 degrees following different periods of incubation at 40 degrews. The optimal temperature for replicase activity in vitro 35 degrees, and greater activity occurred at 40 than at 25 degrees. After about 15 min incubation at 40 degrees, in vivo incorporation of 32P into double-stranded TMV RNA became temperature-sensitive. This function could not be reproduced with the in vitro replicase system.
对超适温度对烟草花叶病毒（TMV）RNA复制酶的影响进行的研究表明，无论在提取后于体外进行孵育，还是在提取和测定之前于该温度的叶片中进行孵育，该酶活性在40摄氏度时都会迅速下降。复制酶活性的丧失与在40摄氏度下孵育不同时间段后，于25摄氏度下将32P体内掺入TMV RNA的能力下降情况平行。体外复制酶活性的最适温度为35摄氏度，且在40摄氏度时比在25摄氏度时具有更高的活性。在40摄氏度下孵育约15分钟后，将32P体内掺入双链TMV RNA变得对温度敏感。这种功能无法用体外复制酶系统重现。
