Filliatreau G, Di Giamberardino L
J Neurochem. 1982 Oct;39(4):1033-7. doi: 10.1111/j.1471-4159.1982.tb11493.x.
We studied the axonal transport characteristics of major cytoskeletal proteins: tubulin, the 69,000 molecular weight protein of chicken neurofilaments, and actin. After intracerebral injection of [35S]methionine, we monitored the specific radioactivity of these proteins as they passed through a very short nerve segment of the chicken oculomotor nerve. Specific radioactivities were assessed by quantitative sodium dodecyl sulfate polyacrylamide gel electrophoresis and autoradiography. The transport patterns obtained for tubulin and the neurofilament protein were very similar, corresponding to transport rate ranges of 1-15 and 1-10 mm/day, respectively. A narrower velocity range of 3 to 4.3 mm/day was found for actin. Tubulin and the neurofilament protein appeared to be largely dispersed during the course of their transit along the nerve. The radioactivity associated with the proteins studied persisted in the nerve segment for a long time after the bulk of the labeled molecules had swept down. Finally, none of these proteins was observed to be transported with the fast axonal transport.
微管蛋白、鸡神经丝分子量为69,000的蛋白以及肌动蛋白。在脑内注射[35S]甲硫氨酸后，我们监测了这些蛋白通过鸡动眼神经的一个非常短的神经节段时的比放射性。通过定量十二烷基硫酸钠聚丙烯酰胺凝胶电泳和放射自显影评估比放射性。微管蛋白和神经丝蛋白的运输模式非常相似，分别对应于1 - 15毫米/天和1 - 10毫米/天的运输速率范围。肌动蛋白的速度范围较窄，为3至4.3毫米/天。微管蛋白和神经丝蛋白在沿神经运输过程中似乎大部分是分散的。在大部分标记分子向下移动后，与所研究蛋白相关的放射性在神经节段中持续了很长时间。最后，未观察到这些蛋白中有任何一种通过快速轴突运输进行运输。
