关于人绝经期促性腺激素诱导小母牛超数排卵的临床及内分泌学研究。

Clinical and endocrinological investigations on superovulation induced in heifers by human menopausal gonadotrophin.

作者信息

Lauria A, Genazzani A R, Oliva O, Inaudi P, Cremonesi F, Monittola C, Aureli G

出版信息

J Reprod Fertil. 1982 Sep;66(1):219-25. doi: 10.1530/jrf.0.0660219.

DOI:10.1530/jrf.0.0660219

Abstract

After an i.v. injection of human menopausal gonadotrophin (hMG), three components of the disappearance time in heifers were found for immunoreactive LH (half-life 13.8--1020 min) and immunoreactive FSH (half-life 21.3--1090 min). When heifers were treated daily with hMG from Days 9, 10 or 11 of the cycle for 3 or 5 days, a total dose of 1350 i.u. FSH induced 10.2 +/- 2.5 (mean +/- s.d.) corpora lutea (CL) in 6 animals and 13 persistent follicles in 4 animals, while a total dose of 2100 i.u. FSH induced 14.3 +/- 1.5 CL in 6 heifers and there were no persistent follicles. Nine heifers treated with a single i.m. injection of 1500 i.u. PMSG exhibited 11.4 +/- 8.6 CL with 17 persistent follicles in 7 animals. Progesterone concentrations rose significantly faster and the oestradiol drop was more rapid after oestrus in heifers treated with hMG than in those treated with PMSG. These results demonstrate that stimulation of the ovarian follicles of heifers is more homogeneous when hMG is used.

摘要

静脉注射人绝经期促性腺激素（hMG）后，发现小母牛体内免疫反应性促黄体生成素（LH）（半衰期13.8 - 1020分钟）和免疫反应性促卵泡素（FSH）（半衰期21.3 - 1090分钟）的消失时间存在三个组分。当在周期的第9、10或11天开始，每天给小母牛注射hMG，持续3或5天，总剂量1350国际单位的FSH在6头动物中诱导出10.2±2.5（平均值±标准差）个黄体（CL），在4头动物中诱导出13个持续性卵泡，而总剂量2100国际单位的FSH在6头小母牛中诱导出14.3±1.5个CL，且无持续性卵泡。9头小母牛单次肌肉注射1500国际单位孕马血清促性腺激素（PMSG）后，表现出11.4±8.6个CL，7头动物中有17个持续性卵泡。与用PMSG处理的小母牛相比，用hMG处理的小母牛发情后孕酮浓度上升明显更快，雌二醇下降更迅速。这些结果表明，使用hMG时，对小母牛卵巢卵泡的刺激更均匀。