Shiomi T, Hieda-Shiomi N, Sato K
Mutat Res. 1982 Aug;95(2-3):313-25. doi: 10.1016/0027-5107(82)90267-6.
Three mutagen-sensitive mutants, MS-1, M10 and Q31, have been isolated from mouse L5178Y cells. MS-1 cells are sensitive to methyl methanesulfonate (MMS), M10 cells are cross-sensitive to X-rays, MMS and 4-nitroquinoline 1-oxide (4NQO), and Q31 cells are cross-sensitive to UV and 4NQO. Lines resistant to 6-thioguanine (TGr) and 5-bromo-2'-deoxyuridine (BUr) were isolated from L5178Y and these three mutagen -sensitive mutants. All the TGr lines were sensitive to 5-bromo-2'-deoxyuridine and HAT medium and all the BUr lines were sensitive to 6-thioguanine and HAT medium. The hybrids homozygous for the mutagen-sensitive markers showed nearly the same sensitivity to UV, 4NQO, X-rays and MMS as their parental TGr and BUr lines. The hybrids constructed by fusing L5178Y BUr and TGr lines from each of MS-1, M10 and Q31 displayed the normal UV, X-ray and MMS resistancy of L5178Y cells. Thus the UV-, X-ray- and MMS-sensitive markers in MS-1, M10 and Q31 were recessive in somatic cell hybrids. The 4NQO-sensitive phenotype, however, behaved codominantly in somatic cell hybrids.
已从小鼠L5178Y细胞中分离出三个对诱变剂敏感的突变体，即MS-1、M10和Q31。MS-1细胞对甲基磺酸甲酯（MMS）敏感，M10细胞对X射线、MMS和4-硝基喹啉1-氧化物（4NQO）具有交叉敏感性，而Q31细胞对紫外线和4NQO具有交叉敏感性。从L5178Y细胞以及这三个对诱变剂敏感的突变体中分离出了对6-硫代鸟嘌呤（TGr）和5-溴-2'-脱氧尿苷（BUr）具有抗性的细胞系。所有TGr细胞系对5-溴-2'-脱氧尿苷和HAT培养基敏感，所有BUr细胞系对6-硫代鸟嘌呤和HAT培养基敏感。对诱变剂敏感标记纯合的杂种对紫外线、4NQO、X射线和MMS的敏感性与其亲本TGr和BUr细胞系几乎相同。通过融合来自MS-1、M10和Q31各自的L5178Y BUr和TGr细胞系构建的杂种表现出L5178Y细胞正常的紫外线、X射线和MMS抗性。因此，MS-1、M10和Q31中对紫外线、X射线和MMS敏感的标记在体细胞杂种中是隐性的。然而，对4NQO敏感的表型在体细胞杂种中表现为共显性。
