Robinson S M, Cadwallader J A, Hill P M
J Appl Physiol Respir Environ Exerc Physiol. 1978 Aug;45(2):320-4. doi: 10.1152/jappl.1978.45.2.320.
An animal model for the study of regional lung function is described. In sheep, the bronchus to the right apical lobe (RAL) of the lung arises directly from the trachea. A tracheal divider, inserted under local anesthesia via a permanent tracheostomy, was used to separate the ventilation of the RAL from that of the rest of the lung. Lobar blood flow was estimated from the RAL contribution to the pulmonary clearance of an intravenous bolus of 85Kr. Gas exchange was measured by conventional methods. Expressed as a percentage of the value obtained for the whole lung, lobar expired volume was 14.7 +/- 4.3%, capillary perfusion was 12.3 +/- 4.2%, oxygen uptake was 14.7 +/- 4.9%, and carbon dioxide production was 13.4 +/- 5.5% (mean +/- SD of 25 studies in 11 animals breathing air). The model permits the study of experimental conditions confined to a single lobe of the lung and offers the advantages of an intact chest wall, spontaneous ventilation and an unanesthetized animal.
本文描述了一种用于研究肺局部功能的动物模型。在绵羊中，右肺尖叶（RAL）的支气管直接起源于气管。通过永久气管造口术在局部麻醉下插入气管分隔器，用于将RAL的通气与肺其余部分的通气分开。通过RAL对静脉注射85Kr肺清除率的贡献来估计肺叶血流量。采用传统方法测量气体交换。以全肺所获值的百分比表示，肺叶呼出量为14.7±4.3%，毛细血管灌注为12.3±4.2%，氧摄取为14.7±4.9%，二氧化碳产生为13.4±5.5%（11只呼吸空气的动物进行25项研究的平均值±标准差）。该模型允许研究局限于单肺叶的实验条件，并具有完整胸壁、自主通气和未麻醉动物的优点。
