Hagen I, Brosstad F, Gogstad G O, Solum N O, Korsmo R
Thromb Res. 1982 Jul 1;27(1):77-82. doi: 10.1016/0049-3848(82)90280-8.
Proteins with different electrophoretic properties were precipitated by a monospecific antiserum to platelet factor 4 either as a "line" or as a "peak" precipitate. The "line" form seen on crossed immunoelectrophoresis of whole platelets was retained when immobilized thrombin was included in the intermediate gel. The retention was partially abolished when thrombin had been blocked at the active serine site or at the fibrinogen binding site. The "peak" form seen on analysis of material secreted from platelets passed unaffected through thrombin-Sepharose. It is suggested that platelet factor 4 exists in the platelets in a state different from that observed extracellularly after platelet secretion.
具有不同电泳特性的蛋白质可被抗血小板因子4的单特异性抗血清沉淀为“线状”或“峰状”沉淀物。当在中间凝胶中加入固定化凝血酶时，全血小板交叉免疫电泳上出现的“线状”形式得以保留。当凝血酶的活性丝氨酸位点或纤维蛋白原结合位点被阻断时，这种保留作用部分消失。对血小板分泌物质分析时看到的“峰状”形式不受影响地通过凝血酶-琼脂糖。这表明血小板因子4在血小板中的存在状态与血小板分泌后在细胞外观察到的状态不同。
