使用人DNA聚合酶α和β在紫外线照射的模型模板上进行DNA合成：引物滑动以解释产物中明显的跨二聚体连续性。

DNA synthesis on UV irradiated model templates using human DNA polymerases alpha and beta: primer slippage to account for evident transdimer continuity in product.

作者信息

Philippe M, Wang T S, Hanawalt P C, Korn D

出版信息

Biochimie. 1982 Aug-Sep;64(8-9):783-8. doi: 10.1016/s0300-9084(82)80129-6.

DOI:10.1016/s0300-9084(82)80129-6

Abstract

We have studied the comparative behavior of human DNA polymerases alpha and beta on a polynucleotide template of dT100 with dA15 covalently attached at the 3' end to serve as primer when defined numbers of pyrimidine dimers are introduced by UV (254 nm) irradiation. We have obtained the surprising result that with both alpha and beta polymerases the incorporation of labelled dATP is enhanced when the template has been irradiated (maximum value at 1000 J/m2 UV incident dose). In the presence of Mn2+, DNA polymerase beta produces a product size corresponding to full copying of the template whether irradiated or not. In marked contrast DNA polymerase alpha produces only short products on unirradiated strands but full copying of irradiated templates. Evidently both polymerases utilize a much larger fraction of the template pool following UV irradiation.

摘要

我们研究了人DNA聚合酶α和β在dT100多核苷酸模板上的比较行为，该模板在3'端共价连接有dA15作为引物，当通过紫外线（254nm）照射引入特定数量的嘧啶二聚体时。我们得到了令人惊讶的结果，即对于α和β聚合酶，当模板被照射时（在1000J/m2紫外线入射剂量下达到最大值），标记的dATP的掺入都会增强。在Mn2+存在的情况下，无论模板是否被照射，DNA聚合酶β产生的产物大小都对应于模板的完全复制。与之形成鲜明对比的是，DNA聚合酶α在未照射的链上只产生短产物，但能完全复制照射过的模板。显然，两种聚合酶在紫外线照射后都利用了模板库中更大的一部分。

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