Strandvik B, Wikström S A
Eur J Clin Invest. 1982 Aug;12(4):301-5. doi: 10.1111/j.1365-2362.1982.tb02236.x.
Urine was collected in four healthy infants, born after 35-39 weeks of gestation. The collections were made for 24 h starting immediately after birth. Bile acids were extracted, separated into conjugate groups, solvolysed, hydrolysed and quantified by gas-liquid chromatography as methyl ester trimethylsilyl ether derivatives. Identification was made by gas-liquid chromatography-mass spectrometry. Cholic acid was the predominating primary bile acid. Non-sulphated tetrahydroxylated bile acids, tentatively identified as 1,3,7,12- and 3,6,7,12-tetrahydroxycholanoic acids, were present in almost the same amounts as cholic acid. A previously unknown tetrahydroxylated cholanoic acid, which might be 2-hydroxylated hyocholic acid, was found in all infants in similar amounts. 3 beta-Hydroxy-5-cholenoic acid was present in the sulphate fraction in the urine from all infants. Lithocholic acid was not found. Small amounts of allo and 6-hydroxylated bile acids were mainly found in the sulphate fraction. Bile acids hydroxylated in the 1-position were found predominantly in the taurine fraction.
收集了4名妊娠35 - 39周后出生的健康婴儿的尿液。收集工作在出生后立即开始，持续24小时。胆汁酸经提取、分离为共轭基团、进行溶剂解、水解，并通过气液色谱法作为甲酯三甲基硅醚衍生物进行定量。通过气液色谱 - 质谱法进行鉴定。胆酸是主要的初级胆汁酸。初步鉴定为1,3,7,12 - 和3,6,7,12 - 四羟基胆烷酸的非硫酸化四羟基胆汁酸，其含量与胆酸几乎相同。在所有婴儿中均发现一种先前未知的四羟基胆烷酸，其含量相似，可能是2 - 羟基猪去氧胆酸。3β - 羟基 - 5 - 胆烯酸存在于所有婴儿尿液的硫酸盐部分中。未发现石胆酸。少量的别胆酸和6 - 羟基胆汁酸主要存在于硫酸盐部分。在1位羟基化的胆汁酸主要存在于牛磺酸部分。
