Anderson R E, Gay C V, Schraer H
J Histochem Cytochem. 1982 Nov;30(11):1135-45. doi: 10.1177/30.11.6815261.
The histochemical localization of carbonic anhydrase by Hansson's cobalt-salt method was compared with immunocytochemical localization in the proventriculus (glandular stomach), the chorioallantoic membrane, and in articular and growth-plate cartilages from the domestic hen. Numerous differences were observed. Staining was positive by Hansson's method and negative by immunocytochemistry for the submucosal glands of the proventriculus, articular cartilage cells, resting and proliferating cells of the growth plate, nuclei, and intercellular spaces. Red blood cells stained faintly and inconsistently by Hansson's method. Both methods were in agreement for the cytoplasm of the surface mucosal cells of the proventriculus, the cytoplasm of the villus cavity cells in the chorioallantoic membrane, and in hypertrophic cells of growth-plate cartilage. Acetazolamide usually inhibited the histochemical reaction, even in those sites that, according to other methods, did not contain enzyme. Consequently, acetazolamide inhibition appears to be an unreliable control for the histochemical reaction.
采用汉森钴盐法对碳酸酐酶进行组织化学定位，并与家鸡前胃（腺胃）、绒毛尿囊膜以及关节软骨和生长板软骨中的免疫细胞化学定位进行了比较。观察到许多差异。汉森法染色呈阳性，而免疫细胞化学法对前胃黏膜下腺、关节软骨细胞、生长板的静止细胞和增殖细胞、细胞核及细胞间隙染色呈阴性。红细胞经汉森法染色浅且不一致。两种方法在前胃表面黏膜细胞的细胞质、绒毛尿囊膜绒毛腔细胞的细胞质以及生长板软骨肥大细胞方面结果一致。乙酰唑胺通常会抑制组织化学反应，即使在根据其他方法不含该酶的部位也是如此。因此，乙酰唑胺抑制似乎不是组织化学反应的可靠对照。
