Huner N P, Hayden D B
Can J Biochem. 1982 Sep;60(9):897-903. doi: 10.1139/o82-115.
The quaternary structures of ribulose-1,5-bisphosphate carboxylase-oxygenase from cold-hardened and unhardened Puma rye were examined by two-dimensional gel electrophoresis according to the method of O'Farrell. The results indicate that major changes in charge heterogeneity occur in the large subunit of this enzyme during growth at cold-hardening temperatures. The extent of charge heterogeneity decreased upon adaptation of Puma rye to cold-hardening temperatures. In addition to charge heterogeneity, molecular weight heterogeneity was also evident In the large subunit polypeptides of the enzyme from cold-hardened and unhardened Puma rye.
采用奥法雷尔的方法，通过二维凝胶电泳对经抗寒锻炼和未经抗寒锻炼的彪马黑麦中的1,5-二磷酸核酮糖羧化酶加氧酶的四级结构进行了研究。结果表明，在抗寒锻炼温度下生长期间，该酶的大亚基电荷不均一性发生了重大变化。彪马黑麦适应抗寒锻炼温度后，电荷不均一性程度降低。除电荷不均一性外，经抗寒锻炼和未经抗寒锻炼的彪马黑麦的该酶大亚基多肽中也存在明显的分子量不均一性。
