Kopun M, Spring H, Granzow C
FEBS Lett. 1982 Oct 18;147(2):207-10. doi: 10.1016/0014-5793(82)81043-0.
According to Oron et al. [FEBS Lett. (1980) 118, 255-258], nuclear glycogen synthase represents an artifact of preparation in rat liver nuclei. We investigated the nuclei isolated from in vitro growing HD33 ascites cells with exclusively cytoplasmic, and from in vivo growing HD33 Ehrlich-Lettré ascites tumor cells with mainly intranuclear, glycogen deposition. Biochemical and ultracytochemical analyses revealed the complete absence of any contamination of the isolated nuclei by cytoplasmic glycogen particles and associated glycogen synthase activity. The glycogen synthase residing in isolated nuclei of in vivo growing HD33 ascites tumor cells represents a truly nuclear enzyme activity.
根据奥伦等人[《欧洲生物化学学会联合会快报》（1980年）118卷，255 - 258页]的研究，核糖原合酶是大鼠肝细胞核制备过程中的一种假象。我们研究了从体外生长的HD33腹水细胞中分离出的细胞核，其糖原沉积完全在细胞质中，以及从体内生长的HD33艾氏腹水肿瘤细胞中分离出的细胞核，其糖原沉积主要在细胞核内。生化和超细胞化学分析表明，分离出的细胞核完全没有受到细胞质糖原颗粒和相关糖原合酶活性的污染。存在于体内生长的HD33腹水肿瘤细胞分离细胞核中的糖原合酶代表一种真正的核酶活性。
