de Alaniz M J, de Gómez Dumm I N, Brenner R R
Mol Cell Biochem. 1982 Oct 1;48(1):15-8. doi: 10.1007/BF00214817.
The effect of different carbon sources on delta 5 desaturation activity in Minimal Deviation Hepatoma 7288 c (HTC) cells was studied. When HTC cells were incubated in the absence of carbon sources in the medium (fasted cells) the conversion of [1-14C]eicosa-8,11,14-trienoic acid to arachidonic acid increased significantly compared to those cells incubated in S-77 medium (fed cells). This activity was not modified if fasted cells were refed with glucose. However, the activity decreased significantly by the addition of lactalbumin hydrolysate to fasted cells. This effect can not be attributed to individual aminoacids since the addition of them to an aminoacid free medium produced no changes on delta 5 desaturation activity. The incubation of HTC cells with S-77 medium supplemented with glucose, pyruvate or glucose plus pyruvate, showed similar delta 5 desaturation activity to those cells incubated in S-77 medium alone.
研究了不同碳源对最小偏差肝癌7288 c（HTC）细胞中Δ5去饱和活性的影响。当HTC细胞在培养基中无碳源的情况下孵育（饥饿细胞）时，与在S-77培养基中孵育的细胞（喂食细胞）相比，[1-14C]二十碳-8,11,14-三烯酸向花生四烯酸的转化显著增加。如果给饥饿细胞重新喂食葡萄糖，这种活性不会改变。然而，向饥饿细胞中添加乳白蛋白水解物会使活性显著降低。这种作用不能归因于单个氨基酸，因为将它们添加到无氨基酸培养基中对Δ5去饱和活性没有影响。用添加了葡萄糖、丙酮酸或葡萄糖加丙酮酸的S-77培养基孵育HTC细胞，其Δ5去饱和活性与仅在S-77培养基中孵育的细胞相似。
