de Alaniz M J, de Gómez Dumm I N, Brenner R R
Mol Cell Biochem. 1980 Dec 16;33(3):165-70. doi: 10.1007/BF00225290.
The effect of fasting on delta 5 desaturation activity in Minimal Deviation Hepatoma 7288c (HTC) cells and in rat liver microsomes was studied. When HTC cells were incubated in the absence of carbon sources in the medium (fasted cells) the conversion of [1-14C] eicosa-8,11,14-trienoic acid to arachidonic acid increased significantly compared to those cells incubated in complete medium (fed cells). This effect was also shown in rat liver microsomes of rats fasted for 12, 24 and 48 hr. Fasting produced a decrease of the amount of ethanolamine fraction in HTC cells together with a significant increase in the specific activity of the same fraction. Fasting also modified the fatty acid composition of HTC cells and rat liver microsomes. There was observed a relative increase of 18:2 omega 6 acid. The importance of the difference in fatty acid and lipid composition among fasted and fed HTC cells and rat liver microsomes on delta 5 desaturation activity was discussed.
研究了禁食对微小偏离肝癌7288c（HTC）细胞和大鼠肝脏微粒体中Δ5去饱和活性的影响。当HTC细胞在培养基中无碳源的情况下孵育（饥饿细胞）时，与在完全培养基中孵育的细胞（喂食细胞）相比，[1-14C]二十碳-8,11,14-三烯酸向花生四烯酸的转化显著增加。在禁食12、24和48小时的大鼠肝脏微粒体中也显示出这种效应。禁食使HTC细胞中乙醇胺组分的量减少，同时该组分的比活性显著增加。禁食还改变了HTC细胞和大鼠肝脏微粒体的脂肪酸组成。观察到18:2ω6酸相对增加。讨论了饥饿和喂食的HTC细胞以及大鼠肝脏微粒体之间脂肪酸和脂质组成差异对Δ5去饱和活性的重要性。
