Reich M, Affronti L F, Wright G L
Tubercle. 1982 Jun;63(2):99-106. doi: 10.1016/s0041-3879(82)80045-7.
The culture filtrate of Mycobacterium tuberculosis H37Ra was fractionated by ammonium sulphate precipitation, diethyl aminoethyl (DEAE) cellulose-Sephadex A-50 column chromatography, preparative isoelectric focusing and polyacrylamide gel electrophoresis to isolate the most antigenic tuberculoprotein. One protein, designated P2, was isolated and determined by immunodiffusion to be the most immunologically reactive antigen present in H37Ra culture filtrate. This protein had a molecular weight of about 22 000, a sedimentation constant of 2.2S and was present in Seibert's A, B, and C proteins.
结核分枝杆菌H37Ra的培养滤液通过硫酸铵沉淀、二乙氨基乙基（DEAE）纤维素-葡聚糖凝胶A-50柱色谱、制备性等电聚焦和聚丙烯酰胺凝胶电泳进行分级分离，以分离出最具抗原性的结核蛋白。分离出一种名为P2的蛋白质，通过免疫扩散测定其为H37Ra培养滤液中免疫反应性最强的抗原。该蛋白质的分子量约为22000，沉降常数为2.2S，存在于赛伯特A、B和C蛋白中。
