Kadouche J, Darne C, Ketels F, Pouletty P, Crevat D, Kalil J, Najean Y
C R Seances Acad Sci III. 1982 Oct 18;295(6):443-8.
Balb/c Mice were immunized with splenic isoferritin. Spleen cells from immunized Mice were fuzed with SP2O myeloma cells. Four monoclonal antibodies designated respectively M29, M211, M386 and B8 were selected. Various isoferritins were analysed by immunodiffusion (Ouchterlony technic). With these monoclonal antibodies and with Rabbit polyclonal sera: human basic and acidic isoferritins and Horse spleen ferritin. Ferritin could be precipitated by these monoclonal antibodies. These results confirm that the ferritin molecule consists of repeating antigenic sites. No immunoreactivity difference could be detected between acidic and basic human ferritine. Species specificity was recognized. The high affinity constant of the M29 monoclonal antibody allowed development of a standardized radioimmunossay of ferritin.
用脾铁蛋白对Balb/c小鼠进行免疫。将免疫小鼠的脾细胞与SP2O骨髓瘤细胞融合。筛选出四种分别命名为M29、M211、M386和B8的单克隆抗体。采用免疫扩散法（欧氏技术）分析各种铁蛋白。使用这些单克隆抗体以及兔多克隆血清：人碱性和酸性铁蛋白以及马脾铁蛋白。这些单克隆抗体可使铁蛋白沉淀。这些结果证实铁蛋白分子由重复的抗原位点组成。未检测到酸性和碱性人铁蛋白之间的免疫反应性差异。识别出物种特异性。M29单克隆抗体的高亲和力常数使得能够开发出标准化的铁蛋白放射免疫测定法。
