Ohashi T, Natori T, Inonata T, Fujimoto Y, Ishikura H, Aizawa M
Gan. 1982 Aug;73(4):606-12.
Five rat leukemia cell lines induced with chemical agents and/or leukemia viruses were investigated in terms of their surface antigens. One of them, KNL-14, which was induced with 1-butyl-1-nitrosourea in WKA rats had RT1-B region-associated antigens on the cell surface. In addition, Thy-1.1 antigens and surface and cytoplasmic mu chains were detected by serological and immunochemical means. From the results it was concluded that this leukemia originated from immature B cells. In the present work, RT1 antigens on the KNL-14 cells were further investigated. The class I and class II antigens were examined and KNL-14 cells were found to have phenotypical antigens and immunogenicity similar to those of B cells from the WKA lymph nodes. As for class II antigens, which are responsible for various immunologic functions, two specificities were detected, Ba-1.2 and Ba-2.7. These KNL-14 cells should be useful for studying the process of rat B cell differentiation and for immunochemical studies of the rat MHC gene products.
对5种用化学试剂和/或白血病病毒诱导产生的大鼠白血病细胞系的表面抗原进行了研究。其中之一，KNL - 14，是在WKA大鼠中用1 - 丁基 - 1 - 亚硝基脲诱导产生的，其细胞表面具有与RT1 - B区域相关的抗原。此外，通过血清学和免疫化学方法检测到了Thy - 1.1抗原以及表面和细胞质的μ链。从这些结果可以得出结论，这种白血病起源于未成熟的B细胞。在本研究中，对KNL - 14细胞上的RT1抗原进行了进一步研究。检测了I类和II类抗原，发现KNL - 14细胞具有与WKA淋巴结B细胞相似的表型抗原和免疫原性。至于负责各种免疫功能的II类抗原，检测到了两种特异性，Ba - 1.2和Ba - 2.7。这些KNL - 14细胞对于研究大鼠B细胞分化过程以及大鼠MHC基因产物的免疫化学研究应该是有用的。
