非抑制性胰岛素样蛋白对肾小球蛋白合成的影响。

Effect of nonsupressible insulin-like protein on glomerular protein synthesis.

作者信息

Cohen M P, Poffenbarger P L, Klein C V, Rye D L

出版信息

J Lab Clin Med. 1978 Sep;92(3):408-14.

Abstract

Partially purified preparations of NSILP were evaluated for effect on incorporation of labeled precursors into protein, collagen, and basement membrane by isolated rat renal glomeruli. Dowex-adsorbed NSILP (0.5 mU/mg) inhibited the secretion of protein and collagen and resulted in a significant diminution of incorporation of labeled lysine into glomerular basement membrane. Glomerular protein and collagen secretion were also inhibited in the presence of Sephadex G-200 NSILP (4.5 mU/mg), but an effect on incorporation of radiolabeled proline, hydroxyproline, lysine, and hydroxylysine into glomerular basement membrane was not observed. The in vitro response of renal glomeruli to NSILP constitutes another "insulin-like" effect of this substance.

摘要

对部分纯化的非特异性胰岛素样多肽（NSILP）制剂进行了评估，以研究其对分离的大鼠肾小球将标记前体掺入蛋白质、胶原蛋白和基底膜的影响。经Dowex吸附的NSILP（0.5 mU/mg）抑制了蛋白质和胶原蛋白的分泌，并导致标记赖氨酸掺入肾小球基底膜的量显著减少。在存在Sephadex G - 200 NSILP（4.5 mU/mg）的情况下，肾小球蛋白质和胶原蛋白的分泌也受到抑制，但未观察到其对放射性标记的脯氨酸、羟脯氨酸、赖氨酸和羟赖氨酸掺入肾小球基底膜的影响。肾小球对NSILP的体外反应构成了该物质的另一种“胰岛素样”效应。