从十二烷基硫酸钠-聚丙烯酰胺凝胶中对放射性标记免疫球蛋白进行电微洗脱和微指纹分析。
Electromicroelution and microfingerprinting of radiolabeled immunoglobulins from sodium dodecyl sulfate-polyacrylamide gels.
作者信息
Boned A, Issa G, Bourgois A
出版信息
J Immunol Methods. 1982 Nov 26;55(1):99-105. doi: 10.1016/0022-1759(82)90082-5.
PMID:6818282
Abstract
An apparatus for electromicroelution from gel slices is described. After 20 min 70-90% of radiolabeled molecules can be extracted from the gel and recovered in 100 mul of buffer. The extracted molecules could be analysed in SDS-PAGE after reduction or could be split to perform microfingerprints (4 cm X 4 cm). This method has been used in several studies on membrane molecules (Fc gamma receptor, surface IgD, T cell receptor).
摘要
本文描述了一种用于从凝胶切片中进行电洗脱的装置。20分钟后，70 - 90%的放射性标记分子可从凝胶中提取出来，并在100微升缓冲液中回收。提取的分子可在还原后进行SDS - PAGE分析，或可进行分割以进行微指纹分析（4厘米×4厘米）。该方法已用于多项关于膜分子（Fcγ受体、表面IgD、T细胞受体）的研究。
Zotero 插件