Korpela J T
Scand J Clin Lab Invest. 1982 Oct;42(6):529-34.
A method is described for the analysis of faecal free and esterified neutral sterols by high-resolution capillary gas-liquid chromatography (GC). After homogenization and filtration of the sample, free and esterified sterols are separated by reversed-phase Lipidex-5000 chromatography. The esterified sterols are saponified and analysed by GC. The free sterols are further fractionated on a Lipidex-5000 straight-phase column into oxo- and hydroxysterols, respectively. The GC separation of the compounds is achieved with an SE-30 open-tubular silica capillary column. The mean recoveries of the authentic sterols are 90.0% +/- 5.8% (SD) and the mean within-assay coefficients of variations are 3.7% +/- 2.9% (SD). The method permits determination of (A) the main animal sterols (cholesterol, coprostanol, epicoprostanol, cholestanol, coprostanone and cholestanone), (B) the amount of total plant sterols (beta-sitosterol, campesterol, stigmasterol and their metabolic conversion products) and (C) the degree of esterification of neutral sterols. In addition results obtained in faecal samples from eight healthy young men eating normal Finnish food are presented.
描述了一种通过高分辨率毛细管气液色谱法（GC）分析粪便中游离和酯化中性固醇的方法。样品经匀浆和过滤后，游离和酯化固醇通过反相Lipidex-5000色谱法分离。酯化固醇经皂化后用GC分析。游离固醇在Lipidex-5000正相柱上进一步分别分离为氧代固醇和羟基固醇。化合物的GC分离通过SE-30开管硅胶毛细管柱实现。真实固醇的平均回收率为90.0%±5.8%（标准差），批内变异系数平均值为3.7%±2.9%（标准差）。该方法可用于测定（A）主要动物固醇（胆固醇、粪甾烷醇、表粪甾烷醇、胆甾烷醇、粪甾烷酮和胆甾烷酮），（B）植物固醇总量（β-谷甾醇、菜油甾醇、豆甾醇及其代谢转化产物）的含量，以及（C）中性固醇的酯化程度。此外，还给出了八名食用正常芬兰食物的健康年轻男性粪便样本的分析结果。
