Dobyan D C, Magill L S, Friedman P A, Hebert S C, Bulger R E
Anat Rec. 1982 Nov;204(3):185-97. doi: 10.1002/ar.1092040303.
The presence of carbonic anhydrase activity in rabbit and mouse kidneys was examined using a histochemical procedure with plastic embedded sections stained by the modified version of the cobalt-phosphate method (Hansson, 1967, 1968; Ridderstrale, 1976). Proximal convoluted tubules (S1 and S2 segments) in both species were strongly positive for carbonic anhydrase activity on the membranes of the luminal, lateral, and basal surfaces. The apical cytoplasm beneath the brush border and the nuclei also stained positively for carbonic anhydrase. The S3 segment (pars recta) of the proximal tubule in the rabbit was positive on the luminal membrane, with somewhat less intensity seen on the lateral and basal surfaces. This segment in the mouse was completely negative. The first part of the thin limbs of long-looped nephrons exhibited strong staining in the mouse. Faint luminal staining was present on descending thin limbs of short-looped nephrons in the mouse. In the rabbit, both the medullary and cortical ascending thick segments of the limb of Henle were completely negative. In contrast, the medullary and cortical ascending thick limbs in the mouse kidney showed staining on all plasma membranes. The intercalated cells in the cortical and medullary portion of the collecting tubules stained positively for carbonic anhydrase in both species. The principal cells of the collecting duct in the cortex were negative in the rabbit and faintly positive in the mouse. The principal cells in the upper medullary collecting tubules in both species stained intensely along the luminal, lateral, and basal cell membranes. The papillary collecting ducts were largely negative in both the rabbit and the mouse. Some interstitial cells in the rabbit in the region of the papillary tip were strongly positive. We conclude that there is a marked difference in carbonic anhydrase activity within and between the renal tubular segments of the rabbit and the mouse. In addition, these distinct differences that exist between the two species correlated with known physiological roles in ion transport.
采用组织化学方法，使用经改良的磷酸钴法（Hansson，1967年、1968年；Ridderstrale，1976年）对塑料包埋切片进行染色，检查了兔和小鼠肾脏中碳酸酐酶活性的存在情况。两种动物的近端曲管（S1和S2段）在管腔、侧面和基底表面的膜上碳酸酐酶活性均呈强阳性。刷状缘下方的顶端细胞质和细胞核碳酸酐酶染色也呈阳性。兔近端小管的S3段（直部）在管腔膜上呈阳性，侧面和基底表面的阳性强度稍弱。小鼠的该段则完全呈阴性。长襻肾单位细段第一部分在小鼠中染色强烈。小鼠短襻肾单位降支细段有微弱的管腔染色。在兔中，髓袢升支粗段的髓质部和皮质部均完全呈阴性。相比之下，小鼠肾脏髓质部和皮质部升支粗段在所有质膜上均有染色。两种动物集合管皮质部和髓质部的闰细胞碳酸酐酶染色呈阳性。兔皮质集合管主细胞呈阴性而小鼠呈弱阳性。两种动物髓质上部集合管主细胞沿管腔、侧面和基底细胞膜强烈染色。兔和小鼠的乳头集合管大多呈阴性。兔乳头尖端区域的一些间质细胞呈强阳性。我们得出结论，兔和小鼠肾小管段内及段间的碳酸酐酶活性存在显著差异。此外，这两个物种之间存在的这些明显差异与离子转运中已知的生理作用相关。
