Boucaud-Maitre Y, Hutinel P, Coquelin H, Cloppet H
Ann Biol Clin (Paris). 1982;40(5):591-5.
Growth of delicate or exacting microorganisms needs the maintenance, in the culture medium, of precise and stable physicochemical conditions. In order to improve knowledge of these medium, and specially of their aerobic or anaerobic state, we measured pH, partial pressures for oxygen and carbon dioxide and glucose concentration of nine commercial blood culture media, in their natural conditions of use. Comparative study of experimental results, and study of successive fabrication lots of two of these media (brain-heart and Schaedler) reveals a great variability of physicochemical parameters, and specially of pO2 and glucose concentration, although initial composition of the media has theoretically not changed. It would be desirable that such parameters be better controlled, so as to encourage regularity of composition of blood culture media and, perhaps, their clinical efficiency.
培养娇弱或苛求的微生物需要在培养基中维持精确且稳定的物理化学条件。为了增进对这些培养基的了解，特别是它们的需氧或厌氧状态，我们在其自然使用条件下测量了九种商业血培养培养基的pH值、氧气和二氧化碳的分压以及葡萄糖浓度。对实验结果的比较研究以及对其中两种培养基（脑心浸液培养基和 Schaedler 培养基）连续生产批次的研究表明，尽管培养基的初始成分理论上没有变化，但物理化学参数存在很大差异，特别是pO2和葡萄糖浓度。希望能更好地控制这些参数，以促进血培养培养基成分的规律性，并可能提高其临床效果。
