外源性磷脂酶A2和C对分离胰岛的促胰岛素分泌作用。

Insulinotropic effects of exogenous phospholipase A2 and C in isolated pancreatic islets.

作者信息

Yamamoto S, Nakaki T, Nakadate T, Kato R

出版信息

Eur J Pharmacol. 1982 Dec 17;86(1):121-4. doi: 10.1016/0014-2999(82)90409-5.

PMID:6819152

Abstract

In isolated pancreatic islets of rats, exogenous phospholipase A2 produced concentration-dependent insulin secretion. p-Bromophenacyl bromide-treated enzyme no longer showed the insulinotropic effect. The insulinotropic effect of phospholipase A2 was inhibited by nordihydroguaiaretic acid (NDGA) and 1-phenyl-3-pyrazolidinone (Phenidone) but not by indomethacin. Exogenous phospholipase C also demonstrated a concentration-related insulinotropic effect. The phospholipase C-induced insulin secretion, however, was inhibited by none of the above inhibitors. These results indicate that a lipoxygenase product(s) is involved in the insulinotropic action of phospholipase A2.

摘要

在大鼠分离的胰岛中，外源性磷脂酶A2产生浓度依赖性胰岛素分泌。对溴苯甲酰溴处理的酶不再显示促胰岛素作用。磷脂酶A2的促胰岛素作用被去甲二氢愈创木酸（NDGA）和1-苯基-3-吡唑烷酮（非那宗）抑制，但不被吲哚美辛抑制。外源性磷脂酶C也表现出浓度相关的促胰岛素作用。然而，磷脂酶C诱导的胰岛素分泌不受上述任何一种抑制剂的抑制。这些结果表明，一种脂氧合酶产物参与了磷脂酶A2的促胰岛素作用。