使用甲基纤维素覆盖物对蓝鳃太阳鱼病毒进行蚀斑测定。

Plaque assay of bluegill virus using a methylcellulose overlay.

作者信息

Robin J, Larivière-Durand C, Berthiaume L

出版信息

J Virol Methods. 1982 Dec;5(5-6):351-4. doi: 10.1016/0166-0934(82)90027-1.

PMID:6819306

Abstract

The EFDL strain of Bluegill virus (BGV) has been titrated in BF-2 cells by the plaque method using 1% methylcellulose overlay. Visible plaques, formed 7 days postinfection, ranged in diameter from 0.5 to 1 mm. Dose-response experiments indicated that a single particle initiated the formation of a plaque. The titration of BGV by this new plaque method provided an accurate technique for the determination of virus concentration.

摘要

通过使用1%甲基纤维素覆盖物的噬斑法，在BF - 2细胞中对蓝鳃太阳鱼病毒（BGV）的EFDL毒株进行了滴定。感染后7天形成的可见噬斑直径在0.5至1毫米之间。剂量反应实验表明，单个病毒粒子可引发噬斑的形成。用这种新的噬斑法对BGV进行滴定，为测定病毒浓度提供了一种准确的技术。