用一种新的粘度测定方法检测经3'-甲基-4-二甲基氨基偶氮苯处理的大鼠肝脏DNA的粘度降低情况。
Decreased viscosity of rat-liver DNA treated by 3'-methyl-4-dimethylaminoazobenzene, detected with a new viscometric approach.
作者信息
Parodi S, Balbi C, Taningher M, Pala M, Russo P, Abelmoschi M L, Santi L
出版信息
Mutat Res. 1982 Nov;106(1):91-9. doi: 10.1016/0027-5107(82)90193-2.
PMID:6819481
Abstract
DNA damage induced in vivo by 3'-methyl-4-dimethylaminoazobenzene (3'CH3DAB) was investigated with 2 differently sensitive techniques: the alkaline elution assay and the viscometric measurement of DNA damage. 3'CH3DAB appeared to be falsely negative with the alkaline elution assay, whereas with the viscometric approach, which is about 30-50 times more sensitive, it appeared positive, and the DNA damage was dose-dependent.
摘要
利用两种不同灵敏度的技术研究了3'-甲基-4-二甲基氨基偶氮苯（3'CH3DAB）在体内诱导的DNA损伤：碱性洗脱分析和DNA损伤的粘度测定。碱性洗脱分析显示3'CH3DAB呈假阴性，而粘度测定法的灵敏度高出约30至50倍，显示其呈阳性，且DNA损伤具有剂量依赖性。
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