Gupta B C, Parshad V R, Guraya S S
Vet Parasitol. 1982 Nov;11(2-3):193-202. doi: 10.1016/0304-4017(82)90042-5.
Survival of immature and mature stages of Paramphistomum cervi, collected from the intestine and the rumen of sheep has been studied in in vitro using Tyrode's, Hank's, Eagle's, Stephenson's saline and Hedon--Fleig media. Twenty-five ml quantities of carbon dioxide (CO2)-rich Hedon--Fleig solution at pH 6.7, at 37 degrees C supported the maximum survival of 5 worms of both immature and mature flukes for periods of 9 and 10 days, respectively. Alterations to the pH of the medium, the incubation temperature and the number of worms significantly affected the period of their in vitro survival. The tissues of immature and mature worms did not show any marked histological and histochemical change until 24 h of in vitro survival in Hedon--Fleig solution when the testes of adult worms started undergoing degeneration. There was a progressive depletion of stored lipids and carbohydrates in the parenchymatous tissue during the course of in vitro survival. After the second day of in vitro maintenance, the maturation of the worms' vitelline follicles was affected, and thereafter they started to degenerate. On Day 4, degeneration of some gastrodermal cells, and a ruptured body wall were observed in some worms. Though the degenerative changes in the tissues of worms appeared at 24 h and continued to increase during in vitro maintenance, the worms survived for 9-10 days.
已使用台氏液、汉氏液、伊格尔氏液、斯蒂芬森氏盐水和赫登 - 弗莱格培养基，在体外研究了从绵羊肠道和瘤胃采集的鹿同盘吸虫未成熟和成熟阶段的存活情况。在37摄氏度、pH值为6.7的富含二氧化碳（CO₂）的25毫升赫登 - 弗莱格溶液中，未成熟和成熟吸虫各5条分别能存活9天和10天，存活时间最长。培养基pH值、孵育温度和虫体数量的改变显著影响其体外存活时间。在赫登 - 弗莱格溶液中体外存活24小时之前，未成熟和成熟虫体的组织未显示任何明显的组织学和组织化学变化，此时成虫的睾丸开始退化。在体外存活过程中，实质组织中储存的脂质和碳水化合物逐渐消耗。体外培养第二天后，虫体卵黄滤泡的成熟受到影响，此后开始退化。在第4天，观察到一些虫体的部分胃皮细胞退化，体壁破裂。尽管虫体组织的退化变化在24小时时出现，并在体外培养期间持续增加，但虫体仍存活了9至10天。
