蛋白质热稳定性与抗蛋白酶解能力之间的相关性。
A correlation between protein thermostability and resistance to proteolysis.
作者信息
Daniel R M, Cowan D A, Morgan H W, Curran M P
出版信息
Biochem J. 1982 Dec 1;207(3):641-4. doi: 10.1042/bj2070641.
Abstract
The loss of activity due to proteolysis of purified L-asparaginase and beta-galactosidase from different sources correlates with the thermal instability of the enzymes. A similar correlation is found when populations of soluble proteins from micro-organisms grown at different temperatures are compared for proteolytic susceptibility and thermal stability. It is proposed that there is a general correlation between the thermostability of proteins and their resistance to proteolysis.
摘要
不同来源的纯化L-天冬酰胺酶和β-半乳糖苷酶因蛋白水解导致的活性丧失与酶的热不稳定性相关。当比较在不同温度下生长的微生物的可溶性蛋白质群体的蛋白水解敏感性和热稳定性时，也发现了类似的相关性。有人提出，蛋白质的热稳定性与其抗蛋白水解能力之间存在普遍的相关性。
相似文献
1
A correlation between protein thermostability and resistance to proteolysis.蛋白质热稳定性与抗蛋白酶解能力之间的相关性。
Biochem J. 1982 Dec 1;207(3):641-4. doi: 10.1042/bj2070641.
2
Amino acid residues adjacent to the catalytic cavity of tetramer L-asparaginase II contribute significantly to its catalytic efficiency and thermostability.与四聚体L-天冬酰胺酶II催化腔相邻的氨基酸残基对其催化效率和热稳定性有显著贡献。
Enzyme Microb Technol. 2016 Jan;82:15-22. doi: 10.1016/j.enzmictec.2015.08.009. Epub 2015 Aug 18.
3
Studies on pH and thermal stability of novel purified L-asparaginase from Pectobacterium carotojorum MTCC 1428.胡萝卜软腐果胶杆菌MTCC 1428新型纯化L-天冬酰胺酶的pH值和热稳定性研究
Mikrobiologiia. 2011 May-Jun;80(3):349-55.
4
Proteolysis of beta-galactosidase following SigmaB activation in Bacillus subtilis.枯草芽孢杆菌中SigmaB激活后β-半乳糖苷酶的蛋白水解作用。
Mol Biosyst. 2012 Jun;8(6):1806-14. doi: 10.1039/c2mb25031d. Epub 2012 Apr 17.
5
Cloning, expression, and characterization of L-asparaginase from a newly isolated Bacillus subtilis B11-06.从一株新分离的枯草芽孢杆菌 B11-06 中克隆、表达和鉴定 L-天冬酰胺酶。
J Agric Food Chem. 2013 Oct 2;61(39):9428-34. doi: 10.1021/jf402636w. Epub 2013 Sep 18.
6
Protein engineering of a cold-active beta-galactosidase from Arthrobacter sp. SB to increase lactose hydrolysis reveals new sites affecting low temperature activity.对节杆菌属SB菌株来源的冷活性β-半乳糖苷酶进行蛋白质工程改造以提高乳糖水解能力，揭示了影响低温活性的新位点。
Extremophiles. 2006 Dec;10(6):515-24. doi: 10.1007/s00792-006-0526-z. Epub 2006 May 31.
7
Chemical modification of L-asparaginase from Cladosporium sp. for improved activity and thermal stability.从枝孢菌属中化学修饰 L-天冬酰胺酶以提高其活性和热稳定性。
Prep Biochem Biotechnol. 2014;44(5):433-50. doi: 10.1080/10826068.2013.833110.
8
Engineering thermal stability of L-asparaginase by in vitro directed evolution.通过体外定向进化工程改造L-天冬酰胺酶的热稳定性
FEBS J. 2009 Mar;276(6):1750-61. doi: 10.1111/j.1742-4658.2009.06910.x. Epub 2009 Feb 13.
9
Expression of aggregation-prone recombinant proteins at low temperatures: a comparative study of the Escherichia coli cspA and tac promoter systems.低温下易于聚集的重组蛋白的表达：大肠杆菌cspA和tac启动子系统的比较研究
Protein Expr Purif. 1997 Mar;9(2):211-8. doi: 10.1006/prep.1996.0678.
10
BbgIV Is an Important Bifidobacterium β-Galactosidase for the Synthesis of Prebiotic Galactooligosaccharides at High Temperatures.BbgIV 是一种重要的双歧杆菌β-半乳糖苷酶，可在高温下合成益生元半乳糖低聚糖。
J Agric Food Chem. 2012 Jan 25;60(3):740-8. doi: 10.1021/jf204719w.
引用本文的文献
1
Rapid clearance of achiral small-molecule drugs using de novo-designed proteins and their cyclic and mirror-image variants.利用从头设计的蛋白质及其环状和镜像变体快速清除非手性小分子药物。
Nat Biomed Eng. 2025 May 29. doi: 10.1038/s41551-025-01404-w.
2
Hyperstable Synthetic Mini-Proteins as Effective Ligand Scaffolds.超稳定合成微型蛋白作为有效的配体支架
ACS Synth Biol. 2023 Dec 15;12(12):3608-3622. doi: 10.1021/acssynbio.3c00409. Epub 2023 Nov 27.
3
Predicting Structural Susceptibility of Proteins to Proteolytic Processing.预测蛋白质对蛋白水解加工的结构易感性。
Int J Mol Sci. 2023 Jun 28;24(13):10761. doi: 10.3390/ijms241310761.
4
Structural Dynamics and Activity of B19V VP1u during the pHs of Cell Entry and Endosomal Trafficking.B19V VP1u 在细胞进入和内体运输的 pH 值下的结构动力学和活性。
Viruses. 2022 Aug 30;14(9):1922. doi: 10.3390/v14091922.
5
Synthesis and Studies of Bulky Cycloalkyl α,β-Dehydroamino Acids that Enhance Proteolytic Stability.稠合环烷基 α,β-去氢氨基酸的合成与研究，这些氨基酸可增强蛋白水解稳定性。
Org Lett. 2022 Jul 29;24(29):5329-5333. doi: 10.1021/acs.orglett.2c01962. Epub 2022 Jul 15.
6
Extreme dependence of Chloroflexus aggregans LOV domain thermo- and photostability on the bound flavin species.聚集体绿屈挠菌LOV结构域的热稳定性和光稳定性对结合黄素种类的极度依赖性。
Photochem Photobiol Sci. 2021 Dec;20(12):1645-1656. doi: 10.1007/s43630-021-00138-3. Epub 2021 Nov 18.
7
Towards a streamlined synthesis of peptides containing α,β-dehydroamino acids.迈向含α,β-脱氢氨基酸肽的简化合成。
Tetrahedron Lett. 2021 Jun 22;74. doi: 10.1016/j.tetlet.2021.153175. Epub 2021 May 10.
8
Design of an engineered ACE2 as a novel therapeutics against COVID-19.设计一种工程化的血管紧张素转换酶2作为抗2019冠状病毒病的新型疗法。
J Theor Biol. 2020 Nov 21;505:110425. doi: 10.1016/j.jtbi.2020.110425. Epub 2020 Jul 29.
9
Lysosomal protein thermal stability does not correlate with cellular half-life: global observations and a case study of tripeptidyl-peptidase 1.溶酶体蛋白热稳定性与细胞半衰期不相关：整体观察及三肽基肽酶 1 的个案研究。
Biochem J. 2020 Feb 14;477(3):727-745. doi: 10.1042/BCJ20190874.
10
Impact of Dehydroamino Acids on the Structure and Stability of Incipient 3-Helical Peptides.脱氢氨基酸对初始三螺旋肽结构和稳定性的影响
J Org Chem. 2020 Feb 7;85(3):1601-1613. doi: 10.1021/acs.joc.9b02747. Epub 2019 Nov 25.
本文引用的文献
1
PURIFICATION, COMPOSITION, AND MOLECULAR WEIGHT OF THE BETA-GALACTOSIDASE OF ESCHERICHIA COLI K12.大肠杆菌K12 β-半乳糖苷酶的纯化、组成及分子量
J Biol Chem. 1965 Jun;240:2468-77.
2
Purification and properties of a proteolytic enzyme from Bacillus stearothermophilus.嗜热脂肪芽孢杆菌蛋白水解酶的纯化及性质
Arch Biochem Biophys. 1957 Aug;70(2):432-41. doi: 10.1016/0003-9861(57)90131-5.
3
The relative thermostability of cytoplasmic proteins from thermophilic bacteria.嗜热细菌胞质蛋白的相对热稳定性
Arch Biochem Biophys. 1957 Mar;67(1):249-51. doi: 10.1016/0003-9861(57)90267-9.
4
The purification and some properties of a stereospecific D-asparaginase from an extremely thermophilic bacterium, Thermus aquaticus.嗜热栖热菌中一种立体特异性D-天冬酰胺酶的纯化及其某些性质
Biochem J. 1982 Jun 1;203(3):787-90. doi: 10.1042/bj2030787.
5
Purification and some properties of an extracellular protease (caldolysin) from an extreme thermophile.来自极端嗜热菌的一种细胞外蛋白酶（嗜热解蛋白）的纯化及某些特性
Biochim Biophys Acta. 1982 Aug 10;705(3):293-305. doi: 10.1016/0167-4838(82)90251-5.
6
Induction and characterization of -galactosidase in an extreme thermophile.嗜热栖热菌中β-半乳糖苷酶的诱导与特性研究
J Bacteriol. 1972 May;110(2):691-8. doi: 10.1128/jb.110.2.691-698.1972.
7
Genetic and enzymatic experiments relating to the tertiary structure of beta-galactosidase.与β-半乳糖苷酶三级结构相关的遗传学和酶学实验。
J Bacteriol. 1968 Nov;96(5):1711-7. doi: 10.1128/jb.96.5.1711-1717.1968.
8
Intracellular protein degradation in mammalian and bacterial cells.哺乳动物细胞和细菌细胞中的细胞内蛋白质降解
Annu Rev Biochem. 1974;43(0):835-69. doi: 10.1146/annurev.bi.43.070174.004155.
9
Anaerobic-nitrate, symbiotic and aerobic growth of Rhizobium japonicum: effects on cytochrome P 450 , other haemoproteins, nitrate and nitrite reductases.日本根瘤菌的厌氧-硝酸盐、共生及需氧生长：对细胞色素P 450、其他血红素蛋白、硝酸盐和亚硝酸盐还原酶的影响。
Biochim Biophys Acta. 1972 Sep 20;275(3):347-54. doi: 10.1016/0005-2728(72)90215-0.
10
The conformation of thermolysin.嗜热菌蛋白酶的构象。
J Biol Chem. 1974 Dec 25;249(24):8030-44.
Zotero 插件