Parr E L, Oliver J R, King N J
Diabetes. 1982 Aug;31 Suppl 4:1-10. doi: 10.2337/diab.31.4.s1.
Measurement of the concentration of H-2 complex antigens in the surface membrane of mouse pancreatic beta-cells by a quantitative immunoferritin-labeling technique revealed that the antigens were present in very small amounts on the beta-cells of five strains. A comparison of the labeling densities in the five strains suggested that beta-cells from C57BL/10Sn congenic strains have about half the H-2 antigen density of BALB/c and C3H/HeJ cells. In C57BL/10Sn mice the antigen density on beta-cells was slightly greater than that on erythrocytes, about 20% of that on thymocytes, and about 2.5% of that present on peritoneal macrophages. Intraperitoneal allografts of c57BL/10Sn islets were uniformly rejected by B10.BR/SgSn diabetic recipients only when the islets were accompanied by 10(7) peritoneal lymphoid cells. When transplanted without peritoneal cells, C57BL/10Sn islets were only marginally rejectable. In a group of nine such allografts, three diabetic recipients were permanently cured and three others showed rejection times of about 30 days. Sensitization of the three mice showing permanent cures, using 10(7) allogeneic peritoneal cells at about 40 days after the transplant, did not cause rejection of the allografts. Isogeneic transplantation of cell suspensions from dissociated islets of Langerhans was markedly less effective in controlling diabetes than intact islets, and dissociation did not obviously improve the rate of allograft survival. However, 5/19 diabetic mice receiving allografts of dissociated islet cells showed long-term reversals of diabetes that were unaffected by administration of 10(7) peritoneal cells at about 100 days after the transplant. Recipient mice whose diabetes was reversed by islet allografts and unaffected by specific sensitization had pancreatic insulin concentrations characteristic of diabetic mice. Our reversals of diabetes with untreated islet allografts may be due to the cleanliness of islet preparations obtained with a modified isolation technique, and to the very low density of H-2 complex antigens on C57BL/10Sn beta-cells.
采用定量免疫铁蛋白标记技术对小鼠胰腺β细胞表面膜中H - 2复合体抗原浓度进行测量，结果显示，在五个品系的β细胞中，这些抗原的含量极少。对这五个品系的标记密度进行比较表明，C57BL/10Sn同源近交系的β细胞中H - 2抗原密度约为BALB/c和C3H/HeJ细胞的一半。在C57BL/10Sn小鼠中，β细胞上的抗原密度略高于红细胞，约为胸腺细胞的20%，约为腹腔巨噬细胞的2.5%。只有当C57BL/10Sn胰岛与10⁷个腹腔淋巴细胞一起移植时，B10.BR/SgSn糖尿病受体才会一致地排斥腹腔内的胰岛同种异体移植。当不伴随腹腔细胞进行移植时，C57BL/10Sn胰岛仅轻微可被排斥。在一组九个这样的同种异体移植中，三个糖尿病受体被永久治愈，另外三个的排斥时间约为30天。在移植后约40天，对三只显示永久治愈的小鼠用10⁷个同种异体腹腔细胞进行致敏，并未导致同种异体移植被排斥。来自解离的胰岛细胞悬液的同基因移植在控制糖尿病方面明显不如完整胰岛有效，并且解离并没有明显提高同种异体移植的存活率。然而，19只接受解离胰岛细胞同种异体移植的糖尿病小鼠中有5只出现了糖尿病的长期逆转，这种逆转不受移植后约100天给予10⁷个腹腔细胞的影响。通过胰岛同种异体移植逆转糖尿病且不受特异性致敏影响的受体小鼠具有糖尿病小鼠特有的胰腺胰岛素浓度。我们用未经处理的胰岛同种异体移植逆转糖尿病可能是由于采用改良分离技术获得的胰岛制剂的清洁度，以及C57BL/10Snβ细胞上H - 2复合体抗原的极低密度。
