J Hyg (Lond). 1980 Aug;85(1):51-7. doi: 10.1017/s002217240002708x.
In a multi-laboratory trial, lauryl tryptose mannitol broth (LTMB) and minerals-modified glutamate medium with added tryptophan (MMGM + T) were compared as single tube tests for the confirmation of Escherichia coli in water; the confirmed results were also compared with the production of gas from minerals-modified glutamate medium without added tryptophan (MMGM). LTMB and MMGM + T gave similar gas and indole results with about 90% of the water samples in most of the laboratories. When compared with the 'correct' results as judged by acid and gas production from lactose peptone water and indole from tryptone water, the difference in the rate of false positive reactions between LTMB and MMGM + T was insignificant; but LTMB gave a significantly lower rate of false negative reactions than MMGM + T. Gas production from MMGM without added tryptophan gave significantly higher rates of both false positive and false negative reactions. Lauryl sulphate is therefore a suitable inhibitory surfactant for use in single tube media for the confirmation of E. coli, which can be recommended.
在一项多实验室试验中，比较了月桂基色氨酸甘露醇肉汤（LTMB）和添加色氨酸的矿物质改良谷氨酸培养基（MMGM + T）作为单管试验用于确认水中大肠杆菌的情况；还将确认结果与未添加色氨酸的矿物质改良谷氨酸培养基（MMGM）产气情况进行了比较。在大多数实验室中，约90%的水样中，LTMB和MMGM + T产生的气体和吲哚结果相似。与通过乳糖蛋白胨水产酸产气和胰蛋白胨水产吲哚判断的“正确”结果相比，LTMB和MMGM + T之间假阳性反应率的差异不显著；但LTMB的假阴性反应率明显低于MMGM + T。未添加色氨酸的MMGM产气产生的假阳性和假阴性反应率均显著更高。因此，月桂基硫酸盐是一种适用于单管培养基中确认大肠杆菌的抑制性表面活性剂，可予以推荐。
