Neskovic N M, Labourdette G
Neurosci Lett. 1980 Aug;19(1):109-14. doi: 10.1016/0304-3940(80)90265-7.
Primary cultures enriched in oligodendroglial cells were prepared from dissociated newborn rat brain. Enzymatic study revealed the presence of high specific activities of UDP-galactose:ceramide galactosyltransferase (CGalT) and 2',3'-cyclic nucleotide-3'-phosphohydrolase (CNP). The specific activity of CGalT was twice as high as that of brain. The CGalT activity increased from 16 days in culture, reached a maximum at about 50 days and declined thereafter. The CNP activity reached a maximum after 60-70 days in culture and remained more or less stable in the following period. The results indicate that in oligodendroglial cells in vitro the regulation of CGalT activity proceeds in a manner similar to that of myelinating brain.
从新生大鼠脑部分离制备富含少突胶质细胞的原代培养物。酶学研究显示存在高比活性的UDP-半乳糖：神经酰胺半乳糖基转移酶（CGalT）和2',3'-环核苷酸-3'-磷酸水解酶（CNP）。CGalT的比活性是脑的两倍。CGalT活性在培养16天时开始增加，在约50天时达到最大值，此后下降。CNP活性在培养60 - 70天后达到最大值，并在随后的时期内或多或少保持稳定。结果表明，体外少突胶质细胞中CGalT活性的调节方式与脑髓鞘形成过程中的调节方式相似。
