Melmed S, Odenheimer D, Carlson H E, Hershman J M
In Vitro. 1982 Jan;18(1):35-42. doi: 10.1007/BF02796383.
Five primary human pituitary tumor cell cultures were initiated from adenoma fragments obtained from patients with prolactin-secreting adenomas and acromegaly. Functional cell cultures were maintained and propagated in monolayer or suspension culture for up to 9 months. Optimal cell viability and growth were achieved using Ham's F10 medium enriched with 20% fetal bovine serum, although cells from a patient with acromegaly also grew in serum-free, defined, hormone-containing medium. Bromocriptine (100 ng/ml) did not alter the growth curve of replicating cells derived from a patient with acromegaly. These cells initially secreted 5.5 micrograms human growth hormone/10(6) cells, and hormone production diminished after 6 wk. Prolactin secretion by cells derived from prolactinomas (0.5 to 1.3 micrograms/10(6) cells/24 h) was stimulated by thyrotropin-releasing hormone (10 ng/ml) in two of the cultures. Both dopamine (10 ng/ml) and nickel chloride (1 mM) suppressed PRL secretion. These studies demonstrate that responsive human pituitary tumor cell cultures can be initiated and maintained.
从患有催乳素分泌腺瘤和肢端肥大症患者的腺瘤碎片中开始建立了五种原代人垂体肿瘤细胞培养物。功能性细胞培养物在单层或悬浮培养中维持和传代长达9个月。使用富含20%胎牛血清的Ham's F10培养基可实现最佳细胞活力和生长，不过来自肢端肥大症患者的细胞在无血清、特定的含激素培养基中也能生长。溴隐亭（100 ng/ml）并未改变来自肢端肥大症患者的复制细胞的生长曲线。这些细胞最初分泌5.5微克人生长激素/10⁶个细胞，6周后激素分泌减少。在两种培养物中，促甲状腺激素释放激素（10 ng/ml）刺激来自催乳素瘤的细胞分泌催乳素（0.5至1.3微克/10⁶个细胞/24小时）。多巴胺（10 ng/ml）和氯化镍（1 mM）均抑制催乳素分泌。这些研究表明，可以建立并维持有反应的人垂体肿瘤细胞培养物。
