Van den Heever L W, Katz K W, Te Brugge L A
J S Afr Vet Assoc. 1982 Dec;53(4):233-4.
Following concern about the recent publication indicating the possible ability of Brucella abortus to survive commercial pasteurisation of naturally contaminated milk, such milk was subjected to biological (BT), serological and bacteriological tests. The raw milk was Brucella Milk Ring Test positive and biotype I was isolated from 4/5 BT guinea pigs, the one tested being seropositive to the Rose Bengal Test, and with serum agglutination and complement fixation titres of 160 and 36 international units respectively. After batch (63 degrees C/30 min) and high temperature, short time (72 degrees C/15 sec) pasteurisation, all 10 BT guinea pigs were bacteriologically and serologically negative, indicating that officially approved methods of commercial pasteurisation rendered naturally Brucella-contaminated raw milk safe for consumption.
鉴于近期有出版物指出布鲁氏菌流产亚种可能具有在对天然受污染牛奶进行商业巴氏杀菌处理后存活的能力，遂对该类牛奶进行了生物学（BT）、血清学和细菌学检测。原料乳的布鲁氏菌乳环试验呈阳性，从5只进行BT试验的豚鼠中的4只分离出生物I型，受试豚鼠对玫瑰红试验血清呈阳性，血清凝集效价和补体结合效价分别为160和36国际单位。在进行批量（63摄氏度/30分钟）和高温短时（72摄氏度/15秒）巴氏杀菌处理后，所有10只进行BT试验的豚鼠在细菌学和血清学检测中均呈阴性，这表明官方批准的商业巴氏杀菌方法可使天然受布鲁氏菌污染的原料乳安全可供食用。
