Shiota K, Takahashi M, Suzuki Y
Endocrinol Jpn. 1981 Aug;28(4):521-34. doi: 10.1507/endocrj1954.28.521.
The effect of testosterone propionate (TP) on the recovery of spermatogenesis was compared in 3 types of experimentally induced gonadotropin-deficient rats; 1. hypophysectomized rats, 2. rats actively immunized to LHRH and 3. hypophysectomized and immunized rats bearing a pituitary isograft. In order to immunize animals to LHRH, deamidated LHRH conjugated with BSA (LHRH-BSA) was injected intradermally to male rats 4 times at 2 week intervals and additional booster injections continued once a month. Anti-LHRH titer was the highest on the 12th week and the titer remained at a relatively high levels thereafter. A remarkable decrease in testicular weight and testosterone production was achieved in 10 weeks associated with a drop in serum LH and FSH levels. In long term immunized rat (LIM rats) which had been immunized for more than 3 months, testicular atrophy advanced to a similar extent to that in long term hypophysectomized rats (HX rats). The administration of TP (1 mg/day) sc for 30 days restored spermatogenesis in LIM rats. The simultaneous administration of anti-LH and -FSH sera in addition to TP did not affect the restoration of spermatogenesis in LIM rats. The same TP treatment in HX rats, however, failed to restore spermatogenesis. Spermatogenesis was reinitiated after TP treatment if immunized-hypophysectomized rats received a pituitary isograft. Though the serum PRL level in LIM rats was one fifth of that in normal rats, a 2.5-fold rise in the PRL level was observed after TP treatment. These results suggest that PRL is involved in the process of spermatogenesis.
在3种实验诱导的促性腺激素缺乏大鼠中比较了丙酸睾酮（TP）对精子发生恢复的影响；1. 垂体切除大鼠，2. 主动免疫促黄体生成素释放激素（LHRH）的大鼠，3. 垂体切除并免疫且带有垂体同种异体移植的大鼠。为了使动物对LHRH免疫，将与牛血清白蛋白（BSA）偶联的脱酰胺LHRH（LHRH-BSA）皮内注射给雄性大鼠，每隔2周注射4次，并每月继续进行额外的加强注射。抗LHRH滴度在第12周时最高，此后滴度保持在相对较高水平。10周时睾丸重量和睾酮产生显著下降，同时血清促黄体生成素（LH）和促卵泡生成素（FSH）水平降低。在长期免疫大鼠（LIM大鼠）中，免疫超过3个月，睾丸萎缩程度与长期垂体切除大鼠（HX大鼠）相似。皮下注射TP（1mg/天）30天可恢复LIM大鼠的精子发生。除TP外同时给予抗LH和抗FSH血清并不影响LIM大鼠精子发生的恢复。然而，相同的TP处理在HX大鼠中未能恢复精子发生。如果免疫垂体切除大鼠接受垂体同种异体移植，TP处理后精子发生会重新启动。虽然LIM大鼠的血清催乳素（PRL）水平是正常大鼠的五分之一，但TP处理后PRL水平升高了2.5倍。这些结果表明PRL参与了精子发生过程。
