Rubiolo E R
Acta Physiol Lat Am. 1981;31(3):199-206.
The immunogenicity of two fractions (1 500 F and 10 000 F) from epimastigotes of Trypanosoma cruzi as well as the supernatant from culture media (SF) were studied using hens, rabbits and opossums. For comparative purposes, sera from individuals with chronic Chagas' disease were also used. A similar, positive response was obtained for the fractions in all the animal species studied using indirect hemagglutination test. Supernatants from culture media were the least immunogenic. By double immunodiffusion test, it was possible to detect a positive response to a different number as well as to different antigens in the three animal species, but there was response to a common antigen by all the different animal species. The common antigen called here major, was present in all the fractions assayed. Human sera from individuals chronically infected showed a variable response. When assayed by double immunodiffusion technique, the major antigen could be detected in just a few samples.
利用母鸡、兔子和负鼠研究了克氏锥虫无鞭毛体的两个组分（1500F和10000F）以及培养基上清液（SF）的免疫原性。为作比较，还使用了慢性恰加斯病患者的血清。使用间接血凝试验，在所研究的所有动物物种中，这些组分均获得了相似的阳性反应。培养基上清液的免疫原性最低。通过双向免疫扩散试验，在这三种动物物种中能够检测到对不同数量以及不同抗原的阳性反应，但所有不同动物物种对一种共同抗原均有反应。这里称为主要抗原的共同抗原存在于所有检测的组分中。慢性感染个体的人血清显示出可变反应。通过双向免疫扩散技术检测时，仅在少数样本中可检测到主要抗原。
