3-磷酸甘油醛脱氢酶与完整人类红细胞膜的关联
Association of glyceraldehyde 3-phosphate dehydrogenase with the membrane of the intact human erythrocyte.
作者信息
Keokitichai S, Wrigglesworth J M
出版信息
Biochem J. 1980 Jun 1;187(3):837-41. doi: 10.1042/bj1870837.
Abstract
Intact human erythrocytes were exposed to low concentrations of glutaraldehyde. After washing and subsequent lysis of the cells, glyceraldehyde 3-phosphate dehydrogenase activity is found to be associated with a membrane fraction and cannot be eluted by salt treatment. Lactate dehydrogenase activity is associated with a supernatant fraction under the same conditions. Preincubation of the intact cells under conditions designed to increase internal NADH concentrations, leads to a lower membrane-associated activity of glyceraldehyde 3-phosphate dehydrogenase after lysis.
摘要
完整的人红细胞暴露于低浓度的戊二醛中。在洗涤并随后裂解细胞后，发现3-磷酸甘油醛脱氢酶活性与膜部分相关，并且不能通过盐处理洗脱。在相同条件下，乳酸脱氢酶活性与上清液部分相关。在旨在增加细胞内NADH浓度的条件下对完整细胞进行预孵育，会导致裂解后3-磷酸甘油醛脱氢酶的膜相关活性降低。
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