溶血过程中红细胞释放3-磷酸甘油醛脱氢酶。没有证据表明该酶在完整细胞中与膜有大量结合。
Glyceraldehyde-3-phosphate dehydrogenase release from erythrocytes during haemolysis. No evidence for substantial binding of the enzyme to the membrane in the intact cell.
作者信息
Rich G T, Dawson A P, Pryor J S
出版信息
Biochem J. 1984 Jul 1;221(1):197-202. doi: 10.1042/bj2210197.
Abstract
Glyceraldehyde-3-phosphate dehydrogenase (G3PDH, EC 1.2.1.12) release from haemolysing erythrocytes, and its redistribution between free and membrane-bound states, were studied with a new type of rapid-mixing filtration apparatus. The apparatus is described. The results indicate that the rate of G3PDH appearance in filtrates is determined not only by the enzyme redistribution but also by the kinetics of haemolysis. We have quantified the extent of haemolysis as a function of time, by measuring the amounts of filterable K+ and lactate dehydrogenase. These are cytoplasmic components that are not membrane-bound. When we correct for incomplete haemolysis, extrapolation to zero times indicates that very little G3PDH is membrane-bound in the intact cell.
摘要
利用一种新型快速混合过滤装置，研究了3-磷酸甘油醛脱氢酶（G3PDH，EC 1.2.1.12）从溶血红细胞中的释放情况及其在游离态和膜结合态之间的重新分布。对该装置进行了描述。结果表明，滤液中G3PDH出现的速率不仅取决于酶的重新分布，还取决于溶血动力学。我们通过测量可过滤的K+和乳酸脱氢酶的量，将溶血程度定量为时间的函数。这些是不与膜结合的细胞质成分。当我们校正不完全溶血时，外推至零时间表明，完整细胞中与膜结合的G3PDH非常少。
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