Leblond P F, Shoucri R
J Microsc. 1978 Jul;113(2):161-70. doi: 10.1111/j.1365-2818.1978.tb02459.x.
This article demonstrates that surface and volume measurements of individual human erythrocytes with varying shapes can be obtained from scanning electron micrographic stereopairs using an approach based on established principles of photogrammetry. Instead of calculating the coordinates of several hundred points plotted for each cell, a procedure that proves tedious and time-consuming, we show that a reasonable approximation of cell surface and volume can be achieved from simple geometrical models constructed with a small number of carefully measured points and angles, using a stereocomparator. The values obtained for two normal erythrocytes and for two distorted red cells from a patient with congenital pyruvate kinase deficiency haemolytic anaemia are consistent with available information on the geometry of these cells. Because scanning electron microscopy requires extensive manipulation of the cells, the values obtained cannot be applied to fresh living material, but appear fairly accurate for the purpose of comparison between cells prepared in the same manner.
本文表明，使用基于摄影测量既定原理的方法，可从扫描电子显微镜立体图像对中获取不同形状的单个人类红细胞的表面积和体积测量值。我们证明，无需计算为每个细胞绘制的数百个点的坐标（这一过程既繁琐又耗时），而是使用立体比较仪，通过由少量仔细测量的点和角度构建的简单几何模型，就能合理近似细胞的表面积和体积。从一名先天性丙酮酸激酶缺乏性溶血性贫血患者身上获取的两个正常红细胞和两个变形红细胞的测量值，与关于这些细胞几何形状的现有信息一致。由于扫描电子显微镜需要对细胞进行大量操作，所获得的值不能应用于新鲜的活体材料，但对于以相同方式制备的细胞之间的比较而言，这些值看起来相当准确。
