白细胞分类计数：光镜和电镜检查结果的比较

Differential leucocyte counts: a comparison of results using light and electron microscopy.

作者信息

Gillett R, Hancock P M, Williams D M, Hill M W

出版信息

J Microsc. 1978 Jul;113(2):205-13. doi: 10.1111/j.1365-2818.1978.tb02463.x.

DOI:10.1111/j.1365-2818.1978.tb02463.x

Abstract

A method is described for handling leucocytes from an inflammatory peritoneal exudate prior to electron microscopy, which allows differential counts from ultra-thin sections to be made. The results of counts from ultrathin sections, viewed on the electron microscope, are compared with samples from the same cell populations prepared on a cytocentrifuge and counted by light microscopy. The results from several cell populations of widely different compositions show clearly that with suitable care taken over preparation and orientation, ultrathin sections can yield comparable differential counts to those obtained by standard light microscope procedures. Possible sources of error are discussed and the advantages of ultrastructural counting assessed. The method has a wide application wherever accurate differential counts are required from cell suspensions processed for electron microscopy.

摘要

本文描述了一种在电子显微镜检查之前处理炎性腹膜渗出液中白细胞的方法，该方法可对超薄切片进行分类计数。将在电子显微镜下观察的超薄切片计数结果与在细胞离心涂片机上制备并用光学显微镜计数的相同细胞群体样本进行比较。来自几种组成差异很大的细胞群体的结果清楚地表明，在制备和定向过程中采取适当的小心措施，超薄切片可以产生与标准光学显微镜程序获得的结果相当的分类计数。讨论了可能的误差来源，并评估了超微结构计数的优点。该方法在需要对用于电子显微镜检查的细胞悬液进行准确分类计数的任何地方都有广泛应用。