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使用电子显微镜对干奶牛分泌物、初乳和正常奶牛的乳汁进行细胞群体的鉴定、特性分析和差异计数。

Identification, properties, and differential counts of cell populations using electron microscopy of dry cows secretions, colostrum and milk from normal cows.

作者信息

Lee C S, Wooding F B, Kemp P

出版信息

J Dairy Res. 1980 Feb;47(1):39-50. doi: 10.1017/s0022029900020860.

DOI:10.1017/s0022029900020860
PMID:7372886
Abstract

Differential counts of electron microscope sections of cell pellets isolated from bovine udder secretions showed that no secretory epithelial cells and very few ductal epithelial cells were present at any stage. The predominant cell type was the macrophage in dry and lactating cows or the polymorphonuclear leucocyte (PMNL) in colostrum. Lymphocytes were also present but no plasma cells were found. The macrophages took up polystyrene latex particles (as did the PMNL) and adhered to glass in culture. Neither macrophage- nor PMNL-rich cell suspensions produced any increase in free fatty acid levels when incubated with fresh milk.

摘要

对从牛乳腺分泌物中分离出的细胞沉淀进行电子显微镜切片的差异计数显示,在任何阶段都不存在分泌上皮细胞,导管上皮细胞也极少。主要的细胞类型在干奶期和泌乳期奶牛中是巨噬细胞,在初乳中是多形核白细胞(PMNL)。淋巴细胞也存在,但未发现浆细胞。巨噬细胞摄取聚苯乙烯乳胶颗粒(PMNL 也如此)并在培养中粘附于玻璃。富含巨噬细胞或 PMNL 的细胞悬液与新鲜牛奶一起孵育时,均未使游离脂肪酸水平有任何升高。

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