Henner W D, Rodriguez L O, Hecht S M, Haseltine W A
J Biol Chem. 1983 Jan 25;258(2):711-3.
Gamma irradiation of DNA in aqueous solution produces single strand breaks with two types of 3'-termini. These termini have been identified as a simple phosphoryl group and a glycolate moiety attached to the 3'-phosphoryl group via the 2"-OH of glycolic acid. Irradiation of 5'-end labeled poly(dG) produces compounds that comigrate with authentic 3',5'-dGDP and 2'-deoxyguanosine-3'-(phospho-2"-O-glycolic acid-5'-monophosphate by thin layer chromatography and polyacrylamide gel electrophoresis. Irradiation of poly(dGdC) followed by digestion to 3'-nucleotides produces 2'-deoxyguanosine-3'-(phospho-2"-O-glycolic acid). The presence of glycolate termini at some strand breaks may affect intracellular repair of these lesions.
水溶液中DNA的γ辐射产生具有两种3'-末端类型的单链断裂。这些末端已被鉴定为一个简单的磷酰基和一个通过乙醇酸的2''-OH连接到3'-磷酰基上的乙醇酸部分。对5'-末端标记的聚（dG）进行辐射会产生与真实的3',5'-二磷酸脱氧鸟苷和2'-脱氧鸟苷-3'-（磷酸-2''-O-乙醇酸-5'-单磷酸）在薄层层析和聚丙烯酰胺凝胶电泳中迁移率相同的化合物。对聚（dGdC）进行辐射，然后消化成3'-核苷酸，会产生2'-脱氧鸟苷-3'-（磷酸-2''-O-乙醇酸）。某些链断裂处乙醇酸末端的存在可能会影响这些损伤在细胞内的修复。
