Agardh C D, Rosén I
J Cereb Blood Flow Metab. 1983 Mar;3(1):78-85. doi: 10.1038/jcbfm.1983.10.
Recovery of electroencephalographic activity and somatosensory evoked responses was studied in paralyzed and lightly anesthetized (70% N2O) rats in which profound hypoglycemia had been induced by insulin administration. The duration of severe hypoglycemia was defined as the duration of a flat electroencephalogram (EEG) recording (5, 30, and 60 min, respectively) before restitution with glucose. The restitution period was followed by continuous EEG monitoring and repeated tests for evoked potentials. After 180 min of recovery, the brains were frozen in situ with liquid nitrogen and analyzed for energy metabolism. In accordance with earlier metabolic studies from this laboratory, the recovery after 60 min of severe hypoglycemia was incomplete, with signs of permanent failure of energy metabolism. There was persistent ATP reduction proportional to the duration of the hypoglycemia. The short-term recovery of EEG and sensory evoked responses was proportional to the duration of severe hypoglycemia. The neurophysiological recovery after 5 min of severe hypoglycemia was complete. After 30 min of severe hypoglycemia, the evoked responses recovered but showed a significant prolongation of latency, compared with normal. After 60 min of severe hypoglycemia, no early evoked response and scanty EEG activity were observed. The neurophysiological observations indicate a persistent deficit of synaptic transmission in the somatosensory pathway, including the cortical projection. This can be correlated with neuropathologic changes that are particularly prominent in intermediate cortical layers, as previously shown.
在注射胰岛素诱发严重低血糖的瘫痪且轻度麻醉（70%氧化亚氮）的大鼠中，研究了脑电图活动和体感诱发电位的恢复情况。严重低血糖的持续时间定义为在用葡萄糖恢复之前脑电图（EEG）记录呈平线的持续时间（分别为5、30和60分钟）。恢复阶段之后是持续的脑电图监测和对诱发电位的重复测试。恢复180分钟后，将大脑在原位用液氮冷冻，并分析能量代谢。根据本实验室早期的代谢研究，严重低血糖60分钟后的恢复是不完全的，存在能量代谢永久性衰竭的迹象。ATP持续减少与低血糖持续时间成正比。脑电图和感觉诱发电位的短期恢复与严重低血糖的持续时间成正比。严重低血糖5分钟后的神经生理恢复是完全的。严重低血糖30分钟后，诱发电位恢复，但与正常情况相比，潜伏期显著延长。严重低血糖60分钟后，未观察到早期诱发电位，脑电图活动稀少。神经生理学观察表明，体感通路（包括皮质投射）中突触传递持续存在缺陷。这可能与中间皮质层中特别突出的神经病理变化相关，如先前所示。
