Jackson D C, Howlett G J, Nestorowicz A, Webster R G
J Immunol. 1983 Mar;130(3):1313-6.
The affinity or equilibrium constant between an Fab fragment derived from monoclonal IgG directed against influenza virus neuraminidase was measured as 4.1 X 10(7) M-1. The method, which makes use of an air-driven ultracentrifuge, is simple and uses extremely small amounts (10(-11) mol) of material. Furthermore, interpretation of the data is based on sound theoretical considerations. The technique also allows m.w. of the interacting species to be measured and the stoichiometry of the reaction to be determined.
针对流感病毒神经氨酸酶的单克隆IgG衍生的Fab片段之间的亲和力或平衡常数经测定为4.1×10⁷ M⁻¹。该方法利用空气驱动的超速离心机，操作简单，且使用的材料量极少（10⁻¹¹摩尔）。此外，数据解释基于合理的理论考量。该技术还能测量相互作用物质的分子量并确定反应的化学计量比。
