Heesemann J, Keller C, Morawa R, Schmidt N, Siemens H J, Laufs R
J Infect Dis. 1983 Jan;147(1):107-15. doi: 10.1093/infdis/147.1.107.
One-hundred fifteen strain of Yersinia enterocolitica were examined for plasmids and plasmid-mediated pathogenic properties. Human strains of serotypes O:3 and O:9 harbored plasmids of 46 and 44 megadaltons, respectively, with 90% homology of DNA sequences. The plasmid-mediated properties were calcium dependence, survival in human serum, conjunctivitis provocation in guinea pigs, and O agglutinogens. One strains of serotype O:8 harbored a 42-megadalton plasmid with 75% sequence homology with plasmids of serotypes O:3 and O:9. An additional plasmid-mediated property was lethality for white mice. Filter hybridization of restriction endonuclease-digested plasmid DNA indicated that a 5.6-megadalton fragment of the plasmid of serotype O:8 had virtually no sequence homology with plasmid DNA of serotypes O:3 and O:9 and therefore may be associated with the lethal factor for mice.
对115株小肠结肠炎耶尔森氏菌进行了质粒及质粒介导的致病特性检测。血清型O:3和O:9的人源菌株分别携带46和44兆道尔顿的质粒，其DNA序列同源性为90%。质粒介导的特性包括钙依赖性、在人血清中的存活能力、豚鼠结膜炎激发试验以及O凝集原。一株血清型O:8的菌株携带一个42兆道尔顿的质粒，其与血清型O:3和O:9的质粒序列同源性为75%。另外一个质粒介导的特性是对小白鼠的致死性。限制性内切酶消化的质粒DNA的滤膜杂交表明，血清型O:8质粒的一个5.6兆道尔顿片段与血清型O:3和O:9的质粒DNA几乎没有序列同源性，因此可能与小鼠致死因子有关。
