Guilvout I, Quilici M L, Rabot S, Lesel R, Mazigh D
Unité d'Ecologie Bactérienne, Institut Pasteur, Paris, France.
Appl Environ Microbiol. 1988 Oct;54(10):2594-7. doi: 10.1128/aem.54.10.2594-2597.1988.
The plasmid profile and BamHI restriction pattern of 17 sorbitol-negative and 1 sorbitol-positive French Yersinia ruckeri strain of the American type strain were studied. The 17 sorbitol-negative strains and the American strain harbored a 62-megadalton (MDa) plasmid with an identical BamHI restriction pattern. Southern hybridization indicated that this 62-MDa plasmid is common among these various strains. The sorbitol-positive strain had four plasmid bands (70, 62, 32, and 25 MDa), and there was no comigration of the DNA fragments of these cleaved plasmids with the fragments of the 62-MDa plasmid. Hybridization of these restricted plasmids with the common 62-MDa plasmid showed a weak DNA homology. The Y. ruckeri plasmid (62 MDa) had a different molecular weight than the virulence plasmid (42 to 47 MDa) of the genus Yersinia, and they had different BamHI restriction patterns. Furthermore, no sequence of the Y. ruckeri plasmid DNA was recognized after Southern hybridization when the 47-MDa plasmid of Y. enterocolitica was used as a probe.
研究了17株山梨醇阴性和1株山梨醇阳性的美国型法国鲁氏耶尔森菌菌株的质粒图谱和BamHI限制性酶切图谱。17株山梨醇阴性菌株和美国菌株都含有一个62兆道尔顿（MDa）的质粒，其BamHI限制性酶切图谱相同。Southern杂交表明，这种62-MDa质粒在这些不同菌株中很常见。山梨醇阳性菌株有四条质粒带（70、62、32和25 MDa），这些切割后的质粒的DNA片段与62-MDa质粒的片段没有共迁移现象。这些限制性质粒与常见的62-MDa质粒杂交显示出较弱的DNA同源性。鲁氏耶尔森菌质粒（62 MDa）的分子量与耶尔森菌属的毒力质粒（42至47 MDa）不同，且它们具有不同的BamHI限制性酶切图谱。此外，当用小肠结肠炎耶尔森菌的47-MDa质粒作为探针进行Southern杂交时，未识别出鲁氏耶尔森菌质粒DNA的序列。
