抗胆碱乙酰转移酶单克隆抗体：制备、特异性及免疫组织化学
Monoclonal antibodies to choline acetyltransferase: production, specificity, and immunohistochemistry.
作者信息
Levey A I, Armstrong D M, Atweh S F, Terry R D, Wainer B H
出版信息
J Neurosci. 1983 Jan;3(1):1-9. doi: 10.1523/JNEUROSCI.03-01-00001.1983.
Immunohistochemical localization of choline acetyltransferase (ChAT) in cholinergic neurons has been difficult to achieve because of problems encountered in producing specific antisera. Here we describe the production and characterization of several distinct monoclonal antibodies to ChAT. Each of the monoclonal antibodies exhibits one of three general patterns of cross-species reactions; one pattern shows reactivity limited mainly to bovine ChAT, a second pattern shows reactivity only to ChAT from higher mammals including humans, and the third pattern shows reactivity to ChAT from all mammals tested. The antibodies bound specifically to two closely related bovine proteins of 68,000 and 70,000 daltons using the Western blotting technique. One of the antibodies was used to localize immunohistochemically known cholinergic structures in the rat brain, including motor neurons, basal forebrain neurons, and neostriatal neurons.
由于在制备特异性抗血清时遇到问题，因此难以实现胆碱能神经元中胆碱乙酰转移酶（ChAT）的免疫组织化学定位。在此，我们描述了几种针对ChAT的不同单克隆抗体的制备和特性。每种单克隆抗体呈现出三种一般跨物种反应模式之一；一种模式显示反应性主要限于牛ChAT，第二种模式显示仅对包括人类在内的高等哺乳动物的ChAT有反应性，第三种模式显示对所有测试哺乳动物的ChAT都有反应性。使用蛋白质印迹技术，这些抗体特异性结合了两种紧密相关的、分子量分别为68,000和70,000道尔顿的牛蛋白质。其中一种抗体被用于免疫组织化学定位大鼠脑中已知的胆碱能结构，包括运动神经元、基底前脑神经元和新纹状体神经元。
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