Kurokawa M, Ishida S, Asakawa S, Iwasa S
Jpn J Med Sci Biol. 1978 Apr;31(2):91-103. doi: 10.7883/yoken1952.31.91.
The effect of bacterial endotoxin on the change in peripheral leukocyte population in mice due to the lymphocytosis-promoting factor (LPF) was investigated. Endotoxin affected not only the total leukocyte count but also the leukocyte proportions at any observation time. Both the coefficient and the intercept of regression of the leukocytic response on dose of LPF were modified by endotoxin. Therefore, in a valid biological assay for LPF using the peripheral leukocyte count as a response, a common reference preparation available for any test materials, irrespective of presence or absence of endotoxin, will be impracticable. Two reference preparations were tentatively established, one being a vaccine and the other an LPF preparation containing little endotoxin. A unitage of LP activity was assigned to each reference preparation. The results also showed that an LPF material to be tested for its possible effects on the lymphatic tissues or the reticuloendothelial system should be free from endotoxin.
研究了细菌内毒素对小鼠外周血白细胞群体因淋巴细胞增多促进因子（LPF）而发生变化的影响。内毒素不仅影响总白细胞计数，还影响任何观察时间的白细胞比例。LPF剂量与白细胞反应的回归系数和截距均因内毒素而改变。因此，在以外周血白细胞计数作为反应的LPF有效生物学测定中，无论测试材料是否存在内毒素，都无法使用一种通用的可供任何测试材料使用的参考制剂。初步建立了两种参考制剂，一种是疫苗，另一种是内毒素含量极低的LPF制剂。为每种参考制剂指定了LP活性单位。结果还表明，用于测试其对淋巴组织或网状内皮系统可能影响的LPF材料应不含内毒素。
