The occurrence of gamma-carboxyglutamate in a protein isolated from ox liver mitochondria.
作者信息
Gardemann A, Domagk G F
出版信息
Arch Biochem Biophys. 1983 Feb 1;220(2):347-53. doi: 10.1016/0003-9861(83)90423-x.
PMID:6824328
Abstract
A calcium-binding protein has been isolated from ox liver mitochondria. The purification procedure includes ammonium sulfate fractionation and chromatographies on Sephadex G-100 and DEAE-Sephadex, respectively. The isolated protein contains gamma-carboxyglutamate, has a molecular weight of 59,000, and an isoelectric point of about 3.8. By submitochondrial fractionation it was shown that the calcium-binding protein is located between the inner and outer mitochondrial membranes.
摘要
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