Sato T, Ohnishi S T
Biochim Biophys Acta. 1983 Jan 5;727(1):196-200. doi: 10.1016/0005-2736(83)90384-x.
Under deoxygenated conditions (PO2 = 0 mmHg), sulfhydryl reagents such as N-ethylmaleimide and iodoacetamide had no effect on sickling, but they did inhibit the anti-sickling activity of chlorpromazine. At the same concentration, these sulfhydryl reagents inhibited the cup formation of chlorpromazine in an oxygenated state (PO2 = 143 mmHg). This supports our previous finding that the anti-sickling effect of membrane-interacting compounds is related to their ability to form a cup-shaped red cell.
在缺氧条件下（氧分压 = 0 mmHg），诸如N - 乙基马来酰亚胺和碘乙酰胺之类的巯基试剂对镰变没有影响，但它们确实抑制了氯丙嗪的抗镰变活性。在相同浓度下，这些巯基试剂抑制了氯丙嗪在氧合状态下（氧分压 = 143 mmHg）的杯状形成。这支持了我们之前的发现，即与膜相互作用的化合物的抗镰变作用与其形成杯状红细胞的能力有关。
